KMID : 0545120120220070902
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Journal of Microbiology and Biotechnology 2012 Volume.22 No. 7 p.902 ~ p.906
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Analysis of Expressed Sequence Tags from the Antarctic Psychrophilic Green Algae, Pyramimonas gelidicola
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Jung Woong-Sic
Lee Sung-Gu Kang Se-Won Lee Yong-Seok Lee Jun-Hyuck Kang Sung-Ho Jin Eon-Seon Kim Hak-Jun
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Abstract
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Expressed sequence tags (ESTs) from the Antarctic green algae Pyramimonas gelidicola were analyzed to obtain molecular information on cold acclimation of psychrophilic microorganisms. A total of 2,112 EST clones were sequenced, generating 222 contigs and 219 singletons, and 200 contigs and 391 singletons from control (4oC) and cold-shock conditions (-2oC), respectively. The complete EST sequences were deposited to the DDBJ EST database (http:// www.ddbj.nig.ac.jp/index-e.html) and the nucleotide sequences reported in this study are available in the DDBJ/EMBL/ GenBank. These EST databases of Antarctic green algae can be used in a wide range of studies on psychrophilic genes expressed by polar microorganisms.
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KEYWORD
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Expressed sequence tags (ESTs), Pyramimonas gelidicola, psychrophilic, Antarctic, database
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